Data cannot be saved for long periods. Results must be measured manually and often differ from analyst to analyst. Radiolabeled and fluorescently labeled analytes detected by measuring radioactivity and florescence respectively.
In other words, it would be nice to be able to use the same explanation for both thin layer and paper chromatography. Limitation in the use of paper chromatography? In fact your question is vast. Why is paper chromatography an appropriate technique to use to determine if different pigments are present in a leaf?
Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase.
Explore more Information Types of chromatography There are various different types of chromatography which are used today in different industries and in day to day life.
Rf stands for "ratio of fronts" and is characteristic for any given compound. But one con is that it is not very accurate compared to otherchromatographies such as gas chromatography. An analysis requires a low amount of sample Compare to other chromatography methods paper chromatography is a cheap technique.
The process of chromatography can be used on a T-shirt. Thin layer chromatography is cheap and fast.